phoP [2019-08-28 12:30:36]

two-component response regulator (OmpR family), regulation of phosphate metabolism

Locus: BSU_29110

Isoelectric point: 5.07

Molecular weight: 27.53 kDa

Protein length: 240 aa

Gene length: 723 bp

Function: regulation of phosphate metabolism (phoA, phoB, phoD, resABCDE, tagA-tagB, tagDEF, [tuaA-H])

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Phosphate metabolism
• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Information processing → Regulation of gene expression → Regulators of core metabolism
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 2,977,800 2,978,522

The protein

Protein family

• OmpR family of two-component response regulators

Paralogous protein(s)

• YkoG, YclJ, WalR

Modification

• phosphorylation by PhoR under conditions of phosphate limitation (stimulates DNA-binding activity)

Effectors of protein activity

• phosphorylation stimulates DNA-binding activity

Structure

• 2OQR (from Mycobacterium tuberculosis, 44% identity) PubMed
• 1MVO (receiver domain)

Localization

• cytoplasm (according to UniProt)

Additional information

• information on binding sites can be found in the PRODORIC2 database

Expression and Regulation

Operons

• Genes: phoP-phoR
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon
  • SigE: sigma factor, PubMed, in SigE regulon

  Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • ScoC: repression, PubMed, in ScoC regulon
  • PhoP: activation, due to PhoP~P binding to the 3’end of the PhoP-PhoR operon PubMed, in PhoP regulon

  Regulation

  • carbon catabolite repression (CcpA) PubMed

Biological materials

Mutant

• 1A966 ( phoP::tet), PubMed, available at BGSC
• BKE29110 (phoP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT, downstream forward: _UP4_TATAAACTGGAGGAGCCAAA
• BKK29110 (phoP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT, downstream forward: _UP4_TATAAACTGGAGGAGCCAAA

Labs working on this gene/protein

• Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Reviews

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Regulation of phoP-phoR expression

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Biochemical analyses

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Targets of PhoR

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Other original publications

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